😵‍💫Completely Randomised Design (CRD)

In a plant pathology laboratory, a researcher wants to compare the efficacy of five fungicides on disease control. Since all petri dishes in the lab have identical growing conditions, there is no need for blocking. She simply randomises the five treatments across 20 petri dishes — this is the Completely Randomised Design, the simplest and most straightforward experimental layout.

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• The CRD is the simplest of all the designs. In this design, treatments are allocated at random to the experimental units over the entire experimental material. In case of field experiments, the whole field is divided into a required number of plots equal size and then the treatments are randomized in these plots. Thus the randomization gives every experimental unit an equal probability of receiving the treatment. This means there is no grouping or blocking of experimental units — the entire experimental area is treated as one homogeneous unit.

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• In field experiments there is generally large variation among experimental plots due to soil heterogeneity. Hence, CRD is not preferred in field experiments. Since CRD has no mechanism to account for soil variability (no blocks), the differences caused by uneven soil fertility get mixed into the error term, making it harder to detect real treatment differences.

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• In laboratory experiments and green house studies, it is easy to achieve homogeneity of experimental materials. Therefore, CRD is most useful in such experiments. In a laboratory, conditions like temperature, humidity, and lighting can be tightly controlled, making the experimental units very uniform — exactly the condition CRD requires to work well.

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Layout of CRD

• The placement of the treatments on the experimental units along with the arrangement of experimental units is known as the layout of an experiment. For example, suppose that there are 5 treatments A, B, C, D and E. Each with 4 replications, we need 20 experimental units. Here, since the number of units is 20, a two digit random number of table will be consulted and a series of 20 random numbers will be taken excluding those which are greater than 20.
• Suppose, the random numbers are 4, 18, 2, 14, 3, 7, 13, 1, 6, 10, 17, 20, 8, 15, 11, 5, 9, 12, 16, 19. After this the plots will be serially numbered and the treatment A will be allotted to the plots bearing the serial numbers 4, 18, 2, 14 and so on. This process of using random number tables ensures that the allocation is free from any personal bias of the researcher.

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Statistical analysis

• Let us suppose that there are ‘k’ treatments applied to ‘r’ plots. These can be represented by the symbols as follows:

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Mathematical Model

y_ij = μ + α_i + ξ_ij

• i = 1,2,…k & j = 1,2,…r
• Where, y_ij is the j^th replication of the i^th treatment
  • μ = general mean effect (Common in all designs). This represents the overall average response across all treatments and replications.
  • α_i = the effect due to i^th treatment. This is the deviation of the i-th treatment mean from the overall mean.
  • ξ_ij = error effect. This captures all uncontrolled random variation in the experiment.

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• Source of Variability:
  • Due to treatment
  • Block
  • Error

Notice that in CRD, while the model lists block as a source, there is actually no blocking in practice. The total variation is partitioned into just two components: treatment variation and error variation.

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• The null hypothesis can be verified by applying the ANOVA procedure. The steps involved in this procedure are as follows:

• N — Number of observations

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ANOVA table

• If the calculated value of F > table vale of F, H₀ is rejected. Then the problem is to know which of the treatment means are significantly different. When the F-test tells us that at least one treatment is different, we need a follow-up comparison to identify exactly which treatments differ from each other.

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• For this, we calculate critical difference (CD):
• CD = SED x t — table value for error d.f. at 5% LOS.
• Where,
  • SED = Standard Error of Difference between the Treatments

• The treatment means are arranged first in descending order of magnitude. If the difference between the two-treatment means is less than CD value, it will he declared as non-significant otherwise significant. This pairwise comparison allows us to rank treatments and identify which ones are statistically superior.

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Advantages and disadvantages of CRD

• It is regarded as one-way classification and no-way control or elimination. This means the data is classified only by treatments (one-way), and there is no attempt to control or eliminate any known source of variation through blocking.
• This design is most commonly used in laboratory experiments such as in Ag. Chemistry, plant pathology, and animal experiments where the experimental material is expected to be homogeneous.
• Applied when the experimental material is limited and homogenous, such as the soil in Pot culture experiments. However, in greenhouse experiments care has to be taken with regard to sunshade, accessibility of air along and across the bench before conducting the experiment.

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• Any number of replications and treatments can be used. The number of replications may vary from treatment to treatment. This flexibility in replication is a unique advantage of CRD — in RBD and LSD, every treatment must have the same number of replications.
• The analysis remains simple even if information on some units are missing.
• This design provides maximum number of degrees of freedom for the estimation of error than the other designs. Since there are no blocks, the degrees of freedom that would have gone to blocks in other designs are added to the error, giving a more precise estimate of experimental error (provided the material is homogeneous).

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• The only drawback with this design is that when the experimental material is heterogeneous, the experimental error would be inflated and consequently the treatments are less precisely compared. The only way to keep the experimental error under control is to increase the number of replications thereby increasing the degrees of freedom for error.
• Local control not used in CRD. This is the key distinction from RBD and LSD, which employ blocking to reduce error.
• Error degree of freedom in CRD: N - k. Where N is the total number of observations and k is the number of treatments.

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Applications

• CRD is most useful in laboratory technique and methodological studies. Ex: in physics, chemistry, in chemical and biological experiments, in some greenhouse studies etc.
• CRD is also recommended in situations where an appreciable fraction of units is likely to be destroyed or fail to respond. Since CRD allows unequal replication, losing some units does not create the complications it would in balanced designs like RBD or LSD.
• CRD can be used with both equal and unequal number of repetitions.

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Summary Cheat Sheet