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🎄Plant Growth Hormones — Auxins, Gibberellins, Cytokinins, ABA, and Ethylene

Discovery, physiological effects, and agricultural applications of the five major plant hormones — auxins, gibberellins, cytokinins, abscisic acid, and ethylene with comparison tables

From Field to Lab — The Invisible Architects of Every Crop

In the previous lesson, we defined plant growth — its phases, measurement, and rate patterns. But growth does not happen on its own. It is orchestrated by chemical messengers called plant hormones (phytohormones) that tell cells when to divide, elongate, differentiate, ripen, or die. Without hormones, the growth machinery we studied would have no coordination.

When a tea planter prunes the growing tips to make bushes denser, the hidden logic is auxin — removing the auxin-rich apical bud releases lateral buds from dormancy. When a farmer applies gibberellic acid to seedless grapes, the berries grow larger without pollination. When a fruit seller places unripe bananas alongside a ripe apple in a closed box, the ethylene released by the apple triggers ripening in the bananas. Every agricultural practice from rooting of cuttings to weed control to fruit ripening involves plant hormones.

This lesson covers:

  1. Auxins — discovery, IAA, synthetic auxins (2,4-D), apical dominance, rooting, weed control
  2. Gibberellins — Bakanae disease, GA₃, bolting, malting, seedless grapes
  3. Cytokinins — kinetin, zeatin, cell division, tissue culture ratios
  4. Abscisic acid (ABA) — stress hormone, stomatal closure, dormancy
  5. Ethylene — gaseous hormone, fruit ripening, ethephon, triple response
  6. Growth retardants — Cycocel, Maleic Hydrazide, Paclobutrazol

This is one of the highest-yield topics in plant physiology for competitive exams.


What Are Plant Hormones?

Plant hormones (phytohormones) bridge the gap between the cellular growth processes of the previous lesson and the whole-plant developmental responses we see in the field. They are produced in minute quantities but control every aspect of plant life.

Plant hormones (phytohormones) are organic substances produced in meristematic tissues, translocated to target sites, and active in extremely minute quantities (parts per million).

  • Term “Phytohormone” suggested by Thimann (1948)
  • Plant Growth Regulators (PGR) include both natural hormones and synthetic compounds

Classification

CategoryExamplesGeneral Effect
Growth promotersAuxins, Gibberellins, CytokininsStimulate cell division, enlargement, differentiation
Growth inhibitorsAbscisic acid (ABA), EthyleneRetard growth, induce dormancy, promote senescence

1. Auxins — The “Growth Hormone”

Auxin was the first plant hormone discovered and remains the most studied. It controls cell elongation, root initiation, apical dominance, and tropisms. Its name comes from the Greek auxein (“to grow”). Auxin is unique among plant hormones because it exhibits polar transport — it moves in only one direction (apex to base).

Discovery

YearScientistContribution
1880Charles Darwin & F. DarwinObserved phototropic bending in Phalaris (Canary Grass) coleoptile
1928F.W. WentIsolated auxin from oat coleoptile tips; credited with discovery of auxin
1931Kogl & Haagen-SmitCoined the term “Auxin”
First isolated from human urine
  • Avena Curvature Test (Went’s bioassay): Agar block with auxin placed on decapitated oat coleoptile → degree of curvature proportional to auxin concentration
Diagram of Went's Avena curvature bioassay showing agar block with auxin placed on one side of decapitated oat coleoptile causing bending proportional to auxin concentration
Went’s Avena Curvature Test — the degree of coleoptile bending is proportional to the concentration of auxin in the agar block, providing the first quantitative bioassay for a plant hormone

Key Facts

PropertyDetail
Natural auxinIndole Acetic Acid (IAA)
PrecursorTryptophan (via Shikimic acid pathway)
Nutrient for IAA synthesisZinc (Zn)
TransportPolar (apex → base = basipetal); active, energy-dependent
Highest concentrationColeoptile tip > root tip

Synthetic Auxins

CompoundAbbreviationSpecial Use
Indole-3-Butyric AcidIBARoot induction in cuttings
Naphthalene Acetic AcidNAAFruit thinning, rooting
2,4-Dichlorophenoxyacetic acid2,4-DSelective weedkiller (kills broadleaf weeds); discovered by Pokorny
2,4,5-Trichlorophenoxyacetic acid2,4,5-TUsed as a defoliant
Malic HydrazideMHAnti-auxin property

Free vs Bound Auxins

TypeStatusExample
Free auxinsPhysiologically active, used in metabolismNormal auxin pool
Bound auxinsAttached to enzymes or anti-auxins, inactiveMango — no rooting even with NAA (bound auxin)

Physiological Effects and Agricultural Applications

EffectMechanismAgricultural Application
Apical dominanceAuxin from apical bud inhibits lateral budsPruning in tea gardens releases laterals → bushy growth
Cell divisionStimulates cambial activity and callus formationTissue culture, grafting (strengthens stock-scion union)
Root initiationPromotes adventitious root formationIBA/NAA for rooting stem cuttings
PhototropismAuxin accumulates on shaded side → more growth → bending toward light
Weed control2,4-D at high concentration kills broadleaf weedsSelective herbicide in wheat, rice, maize
ParthenocarpyInduces seedless fruit developmentSeedless watermelon, tomato
Preventing abscissionNAA prevents abscission — detachment of fruit/leaf from plantApple and mango orchards before harvest
Fruit thinningNAA removes excess fruitsApple, citrus orchards

IMPORTANT

Polar transport of auxin is basipetal (apex → base) and requires ATP from aerobic respiration. Under anaerobic conditions, polar transport stops and auxin moves freely. This is unique to auxin among all plant hormones.


2. Gibberellins (GA)

Gibberellins are the stem elongation hormones. While auxin primarily causes cell elongation in coleoptiles, gibberellins dramatically increase internodal length in intact plants. Their discovery came from a plant disease — one of the best stories in plant science.

PropertyDetail
DiscoveryFrom Bakanae disease (foolish seedling) of rice; fungus Gibberella fujikuroi (Fusarium moniliforme)
Discovered byKurosawa (1926)
Most common formGA₃ (Gibberellic acid)
PrecursorKaurene (a diterpene)
Key effectStem elongation (internodal elongation)

Agricultural Applications

ApplicationExample
Break seed/bud dormancyPotato tuber sprouting
Bolting in rosette plantsCabbage, sugarbeet (long day substitute)
Seedless fruit (parthenocarpy)Seedless grapes — GA₃ spray at flowering
Increase sugarcane yieldInternodal elongation → more juice
Malting in barleyGA₃ promotes alpha-amylase production for starch digestion in brewing
Substitute for vernalisationCan replace cold treatment in some crops
Reversal of dwarfismGenetic dwarf plants grow taller with GA₃

TIP

Exam favourite: Gibberellins were discovered from the “foolish seedling” (Bakanae) disease of rice — infected plants grew abnormally tall. The fungus Fusarium moniliforme produces gibberellic acid.


3. Cytokinins

Cytokinins promote cell division (cytokinesis) and work in concert with auxins. The ratio of auxin to cytokinin is the master switch that determines whether a tissue produces roots, shoots, or undifferentiated callus — a principle that underpins all of modern plant tissue culture.

PropertyDetail
DiscoveryKinetin — first cytokinin, discovered from autoclaved herring sperm DNA by Miller (1955). Kinetin is not natural — it is synthetic
Natural cytokininZeatin (from maize endosperm) — first naturally occurring cytokinin
Site of synthesisRoot tips
TransportThrough xylem (upward)
Key effectCell division (cytokinesis)

Agricultural Applications

ApplicationDetail
Delay senescenceKeeps leaves green longer (Richmond-Lang effect)
Promote cell divisionEssential in tissue culture media (with auxin)
Break dormancySeed germination
Auxin:Cytokinin ratioHigh auxin → root formation; High cytokinin → shoot formation (tissue culture)

IMPORTANT

In tissue culture, the auxin to cytokinin ratio determines organogenesis:

  • High auxin : Low cytokinin → Root formation
  • Low auxin : High cytokinin → Shoot formation
  • Equal ratio → Callus growth

4. Abscisic Acid (ABA) — The “Stress Hormone”

The first three hormones (auxin, GA, cytokinin) are growth promoters. ABA is the first growth inhibitor — it acts as the plant’s “emergency brake,” shutting down growth and conserving resources when conditions turn unfavourable. It is central to drought tolerance and seed dormancy.

PropertyDetail
NatureGrowth inhibitor
Also calledStress hormone, Dormin
Key effectStomatal closure under drought (blocks H⁺ excretion from guard cells)

Effects

EffectAgricultural Relevance
Stomatal closureWater conservation during drought
Promotes dormancySeed dormancy, bud dormancy
Inhibits growthCounteracts auxins and gibberellins
Promotes leaf abscissionLeaf fall in deciduous trees
Promotes senescenceAging of plant parts
Proline accumulationABA triggers accumulation of amino acid Proline under stress for osmoprotection

TIP

Mnemonic — “ABA = Always Blocking Activity”: ABA closes stomata, promotes dormancy, inhibits growth, and promotes abscission — it is the “brake pedal” of plant hormones.


5. Ethylene — The “Ripening Hormone”

Ethylene is unique among all plant hormones — it is a gas (C₂H₄). Despite being classified as a growth inhibitor, it has both promotive and inhibitive roles. Its most famous effect is fruit ripening, which is why a single ripe fruit can trigger ripening in an entire batch.

PropertyDetail
NatureGaseous hormone (only gaseous PGR)
PrecursorAmino acid Methionine
BiosynthesisMethionine → SAM → ACC → Ethylene
Commercial formEthephon (Ethrel) — releases ethylene on application
Key effectFruit ripening
Source exampleRipe apple releases ethylene → ripens nearby bananas

Effects and Applications

EffectAgricultural Application
Fruit ripeningArtificial ripening of banana, mango, tomato
AbscissionPromotes leaf and fruit drop (harvest aid in cotton)
SenescenceAccelerates aging
Breaks dormancyPotato tuber sprouting
Triple responseInhibits stem elongation, promotes radial expansion, causes horizontal growth
Feminising effectIncreases female flowers in cucumber
Flower inductionPineapple and Mango — ethephon triggers flowering
Degreening of citrusUniform colour development in oranges
Latex flow in RubberEthephon application increases latex yield

TIP

Ethephon (Ethrel) is a synthetic ethylene releaser. Key uses: fruit ripening, latex flow in rubber, flower induction in pineapple, cotton defoliation.


6. Growth Retardants and Inhibitors

Beyond the five natural hormones, agriculture uses synthetic growth retardants that manipulate plant architecture. These chemicals typically work by blocking gibberellin biosynthesis, reducing stem elongation while allowing root growth and flowering to proceed normally.

ChemicalTrade NameKey Agricultural Use
ChlormequatCycocel (CCC)Prevents crop lodging in wheat — reduces stem elongation by inhibiting GA synthesis
Maleic Hydrazide (MH)Inhibits cell division; sucker suppressant in tobacco; prevents sprouting in stored onions and potatoes
PaclobutrazolCultarReduces vegetative growth; promotes flowering in mango
DaminozideAlar / B-9Promotes compactness in ornamental plants
MorphactinInhibits phototropism and geotropism

TIP

Key exam facts: Cycocel (CCC) = anti-lodging in wheat. Maleic Hydrazide = sucker control in tobacco, sprouting inhibition in onion/potato. Cultar = flowering in mango.


Exam Mnemonics

TIP

“A-G-C-E-A” — The 5 hormones in order: Auxin (cell elongation) → Gibberellin (stem elongation) → Cytokinin (cell division) → Ethylene (ripening) → ABA (stress)

Precursor mnemonic: “T-K-A-M-M” Tryptophan (Auxin) → Kaurene (GA) → Adenine (Cytokinin) → Methionine (Ethylene) → Mevalonic acid (ABA)


Master Comparison — Five Plant Hormones

FeatureAuxinGibberellinCytokininABAEthylene
TypePromoterPromoterPromoterInhibitorInhibitor
Key effectCell elongationStem elongationCell divisionStomatal closureFruit ripening
Natural formIAAGA₃ZeatinABAC₂H₄
PrecursorTryptophanKaureneAdenineMevalonic acidMethionine
Site of synthesisShoot apex, young leavesYoung leaves, root tipsRoot tipsLeaves, root capsAll tissues (esp. ripening fruits)
TransportPolar (basipetal)Non-polarNon-polarNon-polarDiffusion (gas)
DormancyBreaks dormancyBreaks dormancyInduces dormancyBreaks dormancy
SenescenceDelaysDelaysPromotesPromotes
Agricultural useRooting, weed controlSeedless grapes, boltingTissue cultureDrought toleranceFruit ripening

Summary Table — Key Facts at a Glance

FactAnswer
Auxin discovererF.W. Went
Natural auxinIAA
IAA precursorTryptophan
Nutrient for IAAZinc (Zn)
Auxin transportPolar (basipetal)
Selective weedkiller2,4-D
Gibberellin fromBakanae disease of rice
First cytokininKinetin (from herring sperm DNA)
Natural cytokininZeatin (from maize)
Stress hormoneABA
ABA closesStomata
Only gaseous hormoneEthylene
Ethylene key effectFruit ripening
High auxin:cytokinin ratioRoot formation
Low auxin:cytokinin ratioShoot formation
Apical dominance due toAuxin
Parthenocarpy byAuxin or Gibberellin
Auxin term coined byKogl & Haagen-Smit (1931)
2,4-D discovered byPokorny
GA₃ discovered byKurosawa (1926) from Bakanae disease
GA₃ precursorKaurene (diterpene)
Malting in barleyGA₃ promotes alpha-amylase
Cytokinin siteRoot tips; transport via xylem
Ethylene pathwayMethionine → SAM → ACC → Ethylene
Ethephon (Ethrel)Synthetic ethylene; pineapple flowering, rubber latex
ABA triggersProline accumulation under stress
Cycocel (CCC)Anti-lodging in wheat — inhibits GA
Maleic HydrazideSucker control (tobacco); sprouting inhibition (onion/potato)
Paclobutrazol (Cultar)Promotes flowering in mango
Precursor mnemonicT-K-A-M-M (Tryptophan, Kaurene, Adenine, Methionine, Mevalonic acid)

Plant Growth Regulators: Practical Field Applications

How farmers and horticulturists actually USE plant hormones:

ProblemPGR to UseHow It WorksCrop Example
Promote rooting in cuttingsIBA (Indole Butyric Acid)Stimulates adventitious root formationGrape, pomegranate cuttings
Induce flowering in mango off-seasonPaclobutrazol (Cultar)Inhibits GA synthesis → stops vegetative growth → triggers floweringMango, litchi
Prevent lodging in wheatCycocel (CCC)Anti-gibberellin → shorter, stiffer stemsWheat under high N conditions
Ripen fruits uniformly (tomato, banana)Ethephon (Ethrel)Releases ethylene → triggers ripening cascadeBanana (for uniform colour), tomato
Induce flowering in pineappleEthephon or calcium carbideEthylene promotes flowering in bromeliadsPineapple (synchronised harvest)
Prevent sprouting in stored onion/potatoMaleic Hydrazide (MH)Suppresses cell division in budsOnion, potato (pre-harvest spray)
Improve fruit set in tomato (cold weather)PCPA (Para-Chlorophenoxyacetic Acid)Parthenocarpic fruit development without pollinationTomato, brinjal
Promote latex flow in rubberEthephonEthylene increases bark permeabilityRubber trees
Control suckers in tobaccoMaleic HydrazideKills axillary buds without damaging main stemTobacco
Increase sugarcane internode lengthGA₃ (Gibberellic acid)Promotes cell elongation in internodesSugarcane (higher sugar yield per cane)

Exam-critical mnemonic — hormone precursors “T-K-A-M-M”:

  • Tryptophan → Auxin (IAA)
  • Kaurene → Gibberellin (GA₃)
  • Adenine → Cytokinin
  • Methionine → Ethylene (via SAM → ACC)
  • Mevalonic acid → ABA

The 5 classical hormones as a team analogy: Auxin = the builder (cell elongation, root initiation). Gibberellin = the stretcher (stem elongation, seed germination). Cytokinin = the divider (cell division, delay senescence). Ethylene = the ageing signal (ripening, abscission). ABA = the brake (dormancy, stomatal closure under stress). They don’t work alone — it’s the RATIO between them that determines plant behaviour.


Summary Cheat Sheet

FactAnswer
Term “Phytohormone” suggested byThimann (1948)
Darwin’s phototropism experiment plantPhalaris (Canary Grass) coleoptile
Went’s bioassay nameAvena Curvature Test
Auxin first isolated fromHuman urine
IBA is used forRoot induction in cuttings
2,4,5-T is used asDefoliant
Bound auxin exampleMango — no rooting even with NAA
Gibberellin fungus (perfect stage)Gibberella fujikuroi
Gibberellin fungus (imperfect stage)Fusarium moniliforme
GA₃ substitutes forVernalisation (cold treatment)
GA₃ causes bolting inRosette plants (cabbage, sugarbeet)
Kinetin discovered byMiller (1955)
Kinetin sourceAutoclaved herring sperm DNA
Cytokinin synthesised inRoot tips, transported via xylem
Richmond-Lang effectCytokinin delays senescence (keeps leaves green)
Equal auxin:cytokinin ratio producesCallus growth
ABA also calledDormin
ABA mechanism on stomataBlocks H⁺ excretion from guard cells
Ethylene biosynthesis pathwayMethionine → SAM → ACC → Ethylene
Triple response of ethyleneInhibits stem elongation, promotes radial expansion, horizontal growth
Ethylene feminising effect onCucumber (increases female flowers)
Ethephon triggers flowering inPineapple and Mango
Ethephon increases latex inRubber
Daminozide trade nameAlar / B-9
Morphactin inhibitsPhototropism and geotropism
Only hormone with polar transportAuxin
Growth promoter hormonesAuxin, Gibberellin, Cytokinin
Growth inhibitor hormonesABA, Ethylene

TIP

Next: Lesson 05-03 covers Photoperiodism and Vernalisation — how day length and temperature signals interact with these hormones to control the timing of flowering in crops.

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