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🍡Micronutrients: Fe, Mn, Cu, Zn, B, Mo, Cl, Ni & Beneficial Elements

Complete guide to all 8 micronutrients and 6 beneficial elements — functions, deficiency diseases (Khaira, White Bud, Whiptail, Hollow Heart), toxicity, and diagnostic terms for competitive exams

Why Micronutrients Matter: A Farmer’s Perspective

A rice farmer in eastern UP sees reddish-brown spots on 3-4 week old seedlings — Khaira disease, caused by zinc deficiency. A cauliflower grower in Punjab gets hollow stems — boron deficiency. An apple orchardist in Kashmir finds internal cork in his fruits — again boron. Despite using adequate NPK, these farmers lose 20-40% of their potential yield because they ignored trace elements. Micronutrients are needed in tiny amounts, but their absence causes devastating crop losses.


Micronutrient Concentrations at a Glance

NOTE

These are average plant concentrations. Despite being needed in tiny amounts (ppm), each is absolutely essential.

MicronutrientSymbolAverage Plant ConcentrationUptake Form
ChlorineCl100 ppm (0.01%)Cl-
IronFe100 ppmFe2+, Fe3+
ManganeseMn50 ppmMn2+
BoronB20 ppmH3BO3, BO3-
ZincZn20 ppmZn2+
CopperCu6 ppmCu2+
MolybdenumMo0.1 ppmMoO42-
NickelNi0.1-1.0 ppmNi2+

Iron (Fe)

Key Facts

PropertyDetails
Earth crust5% Iron
Primary mineralsOlivine, Pyrite, Hematite, Goethite
pH relationship10-fold increase in Fe availability for each unit decrease in pH
Mobility in plantImmobile — deficiency on younger leaves
Content in plants100 ppm

Functions of Iron

FunctionAgricultural Significance
Biosynthesis of chlorophyllFe is not a part of chlorophyll but is essential for making it
Oxidation-reduction reactionsRegulates respiration, photosynthesis, nitrate and sulphate reduction
Component of porphyrin moleculesCytochromes, hematin, hemes, ferrichrome, leghemoglobin
Component of ferredoxins (Fe-S proteins)Non-heme iron compounds
Constituent of enzymesCytochrome oxidase, catalase, nitrogenase
Component of flavoproteinsFMN, FAD

Chelates

  • From Greek meaning “claw” — chelate molecules wrap around and grip metal ions
  • Soluble organic compounds that keep Fe, Mn, Cu, Zn in soluble form, preventing fixation
  • Natural chelates: products of microbial activity and organic matter degradation
  • Citric acid and oxalic acid from root exudates have chelating properties

Deficiency of Iron

SymptomDetails
Interveinal chlorosis of younger leavesPrincipal veins remain conspicuously green; other portions turn yellow to white
Called “Iron chlorosis” or “Lime-induced chlorosis” ARS Mains-2017Common in calcareous/alkaline soils
Severe deficiency — leaves become “pale white”Sharp distinction between veins and chlorotic areas
Brassica — necrotic terminal buds at early seedling stageGrowing point damage
Reddish-brown necrotic spots along leaf margins in tree cropsYoung shoot damage

Agricultural example: In the calcareous soils of Gujarat (pH > 8), sorghum and groundnut commonly show lime-induced iron chlorosis. Foliar spray of 0.5% FeSO4 + 0.25% citric acid provides quick correction.

Toxicity of Iron

ConditionDetails
Bronzing in riceFe levels > 300 ppm in leaf blade at tillering
MechanismUnder waterlogging, Fe3+ reduces to highly soluble Fe2+ → excessive uptake
Akiochi disease in riceCaused by both H2S toxicity and Fe deficiency

Manganese (Mn)

Key Facts

PropertyDetails
Earth crust1000 ppm
Primary mineralsManganite MnO(OH), Braunite Mn2O3
pH effectLiming decreases Mn availability (precipitates as MnO2)
Content in plants25-500 mg/kg
Mobility in plantImmobile — deficiency on younger leaves

Functions of Manganese

FunctionDetails
Water-splitting enzyme in Photosystem IIWithout Mn, light reactions cannot proceed
Helps in chlorophyll formationEssential for photosynthesis
Helps movement of Iron within plantFe translocation
Constituent of Mn-SOD (superoxide dismutase)Protects cells against superoxide free radicals in mitochondria
Role in TCA cycle (respiration)Oxidative and non-oxidative decarboxylation with NAD

Deficiency of Manganese

SymptomDetails
Interveinal chlorosis of young leavesChlorotic and necrotic spots between veins
Greyish areas near base of younger leavesBecome yellowish to yellow-orange
Chequered appearance of leafPrincipal and smaller veins stay green
Deficiency occurs when pH exceeds 6.5Mn tied up and unavailable

Crop-specific Mn deficiency diseases:

CropDisease Name
OatsGray Specks / Streaks
PeasMarsh Spot
SugarbeetSpeckled Yellow
SugarcanePahala Blight / Streak Disease
AppleLittle Leaf (due to Mn, not Zn in apple)
WheatMore susceptible to root rot disease

TIP

Distinguishing Fe vs. Mn deficiency: Both cause interveinal chlorosis on young leaves. But Mn deficiency shows tan, sunken spots in chlorotic areas and a chequered pattern. Fe deficiency shows a sharper vein-interveinal contrast without sunken spots.

Toxicity of Manganese

  • Crinkle Leaf of cotton — Mn toxicity in highly acid red-yellow soils
  • Also affects tobacco, soybeans, tree fruits, rapeseed on extremely acid soils

Copper (Cu)

Key Facts

PropertyDetails
pH effectCu availability decreases with increasing pH
Content in plants5-20 mg/kg
Uptake formCu2+ (cupric ion)
PoisonousEven in very small excess concentrations

Functions of Copper

FunctionDetails
Essential for synthesis of Vitamin A
Component of plastocyaninEssential for electron transport in photosynthesis
Electron carrier in oxidation-reductionSimilar functions to Fe
Helps in chlorophyll synthesisVia iron utilisation
Involved in SOD production (in stroma)Protects thylakoids
Disease resistanceStrengthens cell walls
Pollen formation and fertilisationEnhances male flower fertility
Ethylene biosynthesisFruit ripening
Indirectly affects nodule formation ICAR JRF 2016In legumes

Deficiency of Copper

More prevalent in crops on peat and muck (organic) soils — these soils bind Cu strongly, leaving little available.

SymptomDetails
White tips, narrow twisted leavesInterveinal chlorotic mottling
Die-back of new growthNecrosis of apical meristems
Pollen sterilityCritical stage: microsporogenesis — poor fruit set
Empty glumes in wheatNo grain formation
Tip drying and bluish-green leaf tips in rice

Crop-specific Cu deficiency diseases:

CropDisease Name
CitrusDie Back, Exanthema, Gummosis, Little Leaf
GuavaFruit cracking, terminal bud Die Back
CerealsReclamation disease, bleaching and withering of young leaves

Agricultural example: In the newly reclaimed peat soils of Kerala’s Kuttanad region, copper deficiency is common in rice. Application of copper sulphate at 5-10 kg/ha corrects the problem.


Zinc (Zn)

NOTE

Zinc deficiency diseases are high-frequency exam topics: Rice (Khaira), Maize (White Bud), Citrus (Frenching/Mottle Leaf), Brinjal/Mango (Little Leaf), Potato (Fern Leaf).

Key Facts

PropertyDetails
pH effectZn availability decreases with increasing pH; forms calcium zincate at high pH
Content in plants20-150 mg/kg; roots contain more Zn than fruits
MobilityLimited mobility in plant; immobile in soil
Most common deficiencyIn neutral and calcareous soils

Functions of Zinc

FunctionDetails
Constituent of 3 enzymes: Alcohol Dehydrogenase, Carbonic Anhydrase, SOD UPPSC 2021Regulates various metabolic reactions
Synthesis of IAA (Auxin)Explains why Zn deficiency causes stunted growth and small leaves
Gibberellic acid metabolism and RNA synthesisGrowth hormone regulation
Photosynthesis, chlorophyll synthesis, protein synthesisBroad metabolic role
Membrane protein stabilisationVia sulfhydryl group binding
Influences P translocationZn deficiency → excessive P translocation → P toxicity

Deficiency of Zinc

General symptoms: interveinal chlorosis, dead spots, dwarfing, rosetting (reduced internodes), chlorotic/brown rusty spots, lower auxin level.

Crop-specific Zn deficiency diseases:

CropDisease NameKey Symptom
RiceKhaira DiseaseReddish-brown pigmentation on 3-4 week seedlings; papery necrotic tissue
MaizeWhite BudLight yellow areas between veins; white necrotic spots; emerging leaves yellow-white
CitrusMottle Leaf / FrenchingMottled chlorosis
Brinjal, Mango, CottonLittle LeafReduced leaf size, shortened internodes
PotatoFern LeafFern-like leaf deformation
Fruit treesRosetteClustering of small leaves at shoot tips

Agricultural example: Khaira disease is one of the most widespread nutritional disorders of rice in India, especially in the alkaline soils of UP and Bihar. Basal application of ZnSO4 at 25 kg/ha prevents it effectively.

Toxicity of Zinc

  • Excess Zn causes Fe deficiency due to cation competition

Boron (B)

NOTE

Boron deficiency diseases are among the most frequently asked topics: Apple (Fruit Cracking, Internal Cork), Cauliflower (Hollow Stem), Sugarbeet (Hollow Heart), Mango (Black Tip), Grape (Hen & Chicken).

Key Facts

PropertyDetails
Only non-metal among micronutrients
Content in plants10-200 mg/kg
Primary mineralTourmaline and borosilicates
Absorption formH3BO3 (boric acid — neutral molecule, unlike other nutrients)
MobilityImmobile in plants — deficiency on terminal buds/youngest leaves

Functions of Boron

FunctionAgricultural Significance
New cell development in meristematic tissueEssential for cell division
Cell wall formation and stabilisation, lignification, xylem differentiationStructural role
Pollen germination and pollen tube growthWithout B, pollination fails → poor fruit/seed set
Translocation of sugars through cellular membranesPrevents sugar polymerisation
Ca metabolism — keeps Ca soluble in cellsRegulator of K/Ca ratio
N and carbohydrate metabolismBroad metabolic role
Water relations — prevents hydration of root tipsStrengthens roots
Disease resistanceAgainst virus, fungi, insects
Nodule formation in legumesImportant for N fixation

Deficiency of Boron

General symptoms: terminal bud death/discolouration, rosetting, cracking/cork formation, shortened internodes.

Crop-specific B deficiency diseases:

CropDisease Name
AppleFruit Cracking, Internal Cork IBPS AFO 2016
PomegranateFruit Cracking
GrapeHen & Chicken Disease (small and large berries mixed)
CauliflowerBrowning / Hollow Stem
SugarbeetHollow Heart / Heart Rot / Brown Heart
MangoNecrosis / Black Tip
TobaccoTop Sickness
CitrusFruit Cracking AFO 2017
OrangeBitter fruits with thickened peels, blackish discolouration
PapayaDeformed fruits
Flowers”Blossom Blast” — flowers wilt, die, persist on tree

Agricultural example: In apple orchards of Kashmir, internal cork causes unmarketable fruits. Foliar spray of 0.2% borax at pink bud stage prevents the problem.

Toxicity of Boron

  • Yellowing of leaf tips and margins → scorching → early leaf drop

Molybdenum (Mo)

Key Facts

PropertyDetails
Unique pH behaviourMo availability increases with increasing pH — only micronutrient that becomes more available as pH rises
Content in plants0.2-2 mg/kg
Called “Nano-nutrient” UPPSC 2021Needed in extremely small quantities
LimingIncreases Mo availability

Functions of Molybdenum

FunctionDetails
Component of nitrate reductaseCatalyses NO3- → NO2-; essential for N utilisation
Component of nitrogenaseEssential for N fixation in legumes
Required by N-fixing organismsAzotobacter, Clostridium, Nostoc, Anabaena
Iron absorption and translocationEssential for Fe metabolism
Phosphate system and ascorbic acid synthesisMetabolic roles

Deficiency of Molybdenum

SymptomDetails
Chlorotic mottling between veinsReddish/purplish discolouration, marginal necrosis
Brassica crops very susceptibleSymptoms develop in 3-4 week old plants
Cabbage/CauliflowerWhiptailLeaves twisted, elongated, browning
CitrusYellow SpotLeaf yellowing
Legumes — symptoms resemble N deficiencyBecause Mo is needed for N fixation in nodules
NO3 accumulation in plantsInhibits N utilisation for protein synthesis

Agricultural example: Cauliflower in the acidic soils of Jharkhand develops Whiptail because low pH reduces Mo availability. Liming the soil (not just applying Mo) is the long-term solution.


Chlorine (Cl)

Key Facts

PropertyDetails
Plant concentration0.2-2.0% (100 ppm average)
Uptake formCl- through roots and aerial parts

Functions of Chlorine

FunctionDetails
O2 evolution in Photosystem IIWorks alongside Mn in water-splitting
Stomata regulationCounter ion for K+ fluxes in guard cells
Disease resistanceIncreases osmotic pressure in cell sap
Turgor production in guard cellsVia osmotic pressure

WARNING

Cl- makes tobacco leaves thick, brittle, and burn unevenly — so KCl (MOP) is avoided for tobacco; K2SO4 (SOP) is preferred. Cl- also interferes with starch formation, so is avoided in rice and potato.

Deficiency of Chlorine

  • Wilted appearance, chlorotic mottling, bronzing, tissue necrosis in tomato
  • Burning of tips and margins, over-wilting, leaf fall

Toxicity of Chlorine

  • Bronze or yellow leaves with scorched margins
  • Most sensitive crops: tobacco, legumes, potato, sugarcane
  • Leaves thicken and roll; storage quality of potato tubers affected

Nickel (Ni)

Key Facts

PropertyDetails
Plant concentration0.1-1.0 ppm
Most recently recognisedEssential micronutrient (added 1987)
Toxicity riskHigh Ni from sewage sludge may induce Zn or Fe deficiency

Functions of Nickel

FunctionDetails
Metal component of urease enzymeWithout Ni, urease cannot function → toxic urea accumulates in leaf tips
Essential for N metabolism
Stimulates nodule weight and crop yield
Needed for grain filling and seed vitality

Beneficial Elements

These are not essential for all plants but significantly boost growth or stress tolerance in specific crops.

ElementKey FunctionsImportant Crops
Silicon (Si)Strengthens cell walls, disease/pest resistance, reduces lodging, drought resistance. Si needed when rice straw < 11% SiRice, Sugarcane, Maize. Freckling in sugarcane = low Si symptom
Cobalt (Co)Component of Vitamin B12; essential for leghemoglobin formation and N2 fixation by RhizobiumLegumes (not essential for the plant itself, but for its N-fixing bacteria)
Sodium (Na)Replaces K in C4/CAM plants; PEP regeneration; osmo-regulation; drought resistanceSugarbeet, Turnip, C4 plants
Vanadium (V)Substitutes for Mo in N fixation; beneficial for microorganism growthGreen algae, N-fixing bacteria
Selenium (Se)Analogous to S; can replace S in methionine, cysteine, cystine synthesis; antioxidantImportant for animal nutrition via forages
Aluminium (Al)Activator of glandular system at low concentration; toxic at higher amounts (damages root tips in acid soils)Tea (stimulated by low Al)

Nutrient Mobility and Absorption Summary

Absorption Speed

SpeedNutrients
RapidUrea Nitrogen, Potassium, Zinc
ModerateCalcium, Sulfate, Manganese, Boron
SlowMagnesium, Copper, Iron, Molybdenum

Mobility in Soil

MobilityNutrients
Highly mobile (prone to leaching)NO3-, SO42-, Cl-, H2BO32-, Mn2+
Moderately mobileNH4+, K, Ca, Mg, Mo
ImmobileOrganic N, P, Cu, Fe, Mn, Zn (chelated forms are mobile)

Mobility in Plant

MobilityNutrientsDeficiency Appears On
Highly mobileN, P, K, Cl, Mo, MgOlder leaves first
Moderately mobileS, Cu, Fe, Mn, ZnNew growth
ImmobileB, CaGrowing tips / youngest leaves

Key Diagnostic Terms

TermMeaningCaused by Deficiency of
ChlorosisYellowing of leavesN, K, Fe, Mg, Mn, Zn, S
MottledColoured spots (anthocyanin) on leaf surfaceN, Mg, P, S
NecrosisPatches of dead tissueMg, K, Zn, Ca, Mo

Summary Table: Micronutrient Deficiency Diseases

CropDiseaseNutrient Deficiency
RiceKhaira DiseaseZn
RiceBronzingFe toxicity
RiceAkiochiH2S toxicity + Fe deficiency
MaizeWhite BudZn
OatsGray SpecksMn
PeasMarsh SpotMn
SugarbeetSpeckled YellowMn
SugarbeetHollow HeartB
SugarcanePahala BlightMn
AppleFruit Cracking / Internal CorkB
CauliflowerHollow Stem / BrowningB
Cauliflower/CabbageWhiptailMo
MangoBlack TipB
GrapeHen & ChickenB
CitrusDie Back / ExanthemaCu
CitrusMottle Leaf / FrenchingZn
CitrusYellow SpotMo
PotatoFern LeafZn
TobaccoTop SicknessB
CottonCrinkle LeafMn toxicity
WheatEmpty GlumesCu
TeaTea YellowsS or Fe
TobaccoSand DrownMg

TIP

Mnemonic for Boron deficiency crops:Apple Cauliflower Sugarbeet Mango Grape Tobacco Citrus” — “A Careful Student Must Get Top Class” = all Boron deficiency!


References
- Tisdale, S.L., Nelson, W.L., Beaton, J.D., Havlin, J.L. 1997. Soil Fertility and Fertilizers. 5th ed. Prentice Hall of India, New Delhi.
- Singh, S.S. 1995. Soil Fertility and Nutrient Management. Kalyani Publishers, Ludhiana.
- Mortvedt, J.J. et al. (Eds.). Micronutrients in Agriculture. No.4, SSSA, Madison, Wisconsin, USA.
- IARI Toppers Soil Science Part-9 (6th Edition 2025).
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